The use of tissue culture method for the study of lichenized fungi (Lobariaceae Chevall., Parmeliaceae Zenker.) of the European part of Russia

Lichenized fungi as a multicomponent biological system are an interesting but difficult research object. In view of the complex interaction myco- and photobiont, slow growth natural thalli, their exceptional exactingness to environmental factors, study characteristics lichens is significantly difficult. This article discusses problem studying anatomical morphological structure photobiont lichenized using tissue culture method on example rare species (Lobaria pulmonaria) with pharmaceutical potential (Usnea dasopoga, Cetraria islandica). The authors proposed for cultivating photobionts these synthetic nutrient media purpose further fungi. Fragments apothecia (for C. islandica), soredia U. dasopoga L. were used donor material. For introduction under sterile conditions, homogenate thallus region was prepared. Pure cultures pulmonaria, islandica obtained three types hormone-free media, namely MS medium, modified medium reduced nitrogen content, Czapek medium. verification results carried out taking into account microscopy photobionts. Using microscopy, dimensional mycobionts determined. diameters hyphae 4.3–4.4 µm, 4.6 4.1–4.3 respectively, which corresponds size in samples. use makes it possible analyze system, well contribute preservation components Red Data Book; while optimizing reduce anthropogenic load populations when them medicinal raw materials.